Cell Division Cycle And Apoptosis Regulator 1 (CCAR1)

CARP1 protein contains a putative cold-shock protein domain expected to bind RNA and a putative DNA-binding motif predicted to be involved in chromosomal organization. Immunohistochemical analysis detected CARP1 in the perinuclear compartment of human breast cancer cells, and the protein had an apparent molecular mass of 130 kD by SDS-PAGE.

Increased CARP1 expression resulted in elevated levels of the cyclin-dependent kinase inhibitor p21 (CDKN1A) and apoptosis. Conversely, reduced CARP1 expression via an antisense cDNA inhibited CD437- or adriamycin- induced apoptosis. CARP1 interacted with 14-3-3 and reduced the expression of cell cycle regulatory genes, including MYC and cyclin B1 (CCNB1).