Cell Division Cycle Associated Protein 5 (CDCA5)

The deduced human and mouse proteins contain 252 and 264 amino acids, respectively. Both contain a KEN box for APC-dependent ubiquitination. Immunofluorescence analysis revealed a punctate nuclear distribution of CDCA5 during interphase and a diffuse distribution of CDCA5 throughout the cell during mitosis. There was no apparent concentration of CDCA5 on chromatin in mitotic cells. The level of CDCA5 dropped in synchronized HeLa cells during interphase.Overexpression of CDCA5 in Xenopus embryos caused failure to resolve and segregate sister chromatids in mitosis and increased the amount of cohesin associated with metaphase chromosomes. In cultured human and mouse cells, depletion of CDCA5 caused mitotic arrest and the complete failure of sister chromatid cohesion.