Centromere Protein M (CENPM)

The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis.

CENPMcontains 180 amino acids. In silico analysis suggested that PANE1 was expressed predominantly in cancer tissues. Dot and Northern blot analyses revealed highest expression of a 1.2-kb transcript in fetal liver, thymus, and spleen, as well as in lymphoid cells and tumor cell lines, including B- and T-cell leukemias. PANE1 was expressed in activated, but not resting, B and T lymphocytes. Immunofluorescence microscopy showed nuclear localization of PANE1 in nonconfluent culture cells, but not in confluent or dividing cells.