Late Endosomal/Lysosomal Adaptor And MAPK And MTOR Activator 1 (LAMTOR1)

p27RF-Rho, was first identified as a protein that copurified with MMP1 from a human melanoma cell line. The protein contains 1 myristoylation and 2 palmitoylation sites at its N terminus. Northern blot analysis detected variable expression of p27RF-Rho mRNA in all human tissues examined. Orthologs of p27RF-Rho were identified in a number of vertebrate species. p27RF-Rho localized to a punctate distribution in a human fibrosarcoma cell line, and Western blot analysis detected p27RF-Rho at an apparent molecular mass of 17 kD.The deduced 161-amino acid protein contains a dileucine motif near its N-terminal end in addition to the N-terminal myristoylation and palmitoylation sites. Confocal microscopy showed that fluorescence-tagged PDRO localized to ring-shaped structures that also expressed markers of late endosomes and lysosomes.