Myelin Regulatory Factor (MYRF)

Ndt80 contains 2 proline-rich sequences, and the C-terminal half contains 2 transmembrane helices. Northern blot analysis detected a 5.7-kb transcript in lung and retinal pigment epithelial tissue and in a retinal pigment epithelial cell line. Minor expression was detected in cerebellum and retina. RT-PCR detected expression in brainstem, uterus, basal ganglion, and liver; no expression was detected in lymphocytes or heart. Examination of EST databases revealed widespread expression and tissue-specific abundance.RT-PCR ELISA detected moderate expression in brain, lung, ovary, and spinal cord, and in all specific brain regions examined except cerebellum. Low expression was detected in liver and pancreas, and little to no expression was found in heart, skeletal muscle, kidney, spleen, and testis.