Coiled Coil Domain Containing Protein 62 (CCDC62)

CCDC62contains 2 N-terminal coiled-coil motifs and 2 C-terminal LxxLL motifs. Transcript-1 differs from transcript-2 only in exon 12, which encodes the last 12 or 15 amino acids of the respective proteins; all other coding regions are identical. Mouse Erap75 shares 50% homology with human ERAP75. Western blot analysis of human cell lines showed highest ERAP75 expression in prostate cancer cell lines, with moderate expression in a lung cancer cell line and an immortalized benign prostate epithelial cell line, and low expression in a breast cancer cell line. RT-PCR showed that Erap75 was widely expressed at variable levels in mouse tissues, with highest expression in prostate. Immunofluorescence analysis revealed colocalization of ERAP75 and ER-beta in nuclei of LNCaP human prostate cancer cells.