Translation Machinery Associated Protein 7 (TMA7)

HSPC016 was confirmed to express in the human dermal papilla cells by means of in situ hybridization and reverse transcriptase-polymerase chain reaction. In order to rudimentarily elucidate its biological function, a recombinant eucaryotic expressing plasmid pcDNA3.1(+)/HSPC016 was constructed and was transfected into the human dermal papilla cells and 3T3 fibroblast cells by means of Nucleofector(TM) technique (Amaxa, Cologne, Germany).

Terminal deoxynucleotidyl transferase-mediated d-UTP nick end Labelling (TUNEL) assay showed that the expression of gene HSPCO16 resulted in the apoptosis of these cells, which suggested that the apoptosis of dermal papilla cells might be associated with the expression of gene HSPC016 in vitro.