Complexin 3 (CPLX3)

The deduced 158-amino acid protein has a calculated molecular mass of 17.6 kD. CPX III contains a C-terminal CAAX box motif for posttranslational prenylation. CPX III shares more than 91% amino acid identity with mouse Cpx III. Northern blot analysis of several mouse and rat tissues detected Cpx III only in retina. EST database analysis indicated mouse Cpx III is expressed in brain and eye. In situ hybridization of mouse brain sections detected Cpx III in pyramidal cells of the hippocampus, granule cells of dentate gyrus and cerebellum, and Purkinje cells of cerebellum. Western blot analysis detected Cpx III in several rat brain regions. Cpx III associated with cytosolic and membrane synaptic fractions of fractionated rat cortex. Immunohistochemical analysis of mouse retina detected Cpx III in ribbon and conventional synapses of rods and cones.