Cordon Bleu Homolog (COBL)

The deduced 1,337-amino acid protein has a calculated molecular mass of 144 kD and shares 67% identity with human COBL. Both proteins contain 3 lysine-, arginine-, and proline-rich (KRAP) motifs in the N-terminal region, 3 Wiskott-Aldrich syndrome protein (WASP) homology domain-2 (WH2) regions near the C terminus, 2 putative N-glycosylation motifs, and potential nuclear localization signals. An alternatively spliced transcript in both mouse and human encodes a COBL protein lacking a short sequence between KRAP motifs 1 and 2. In situ hybridization of mouse embryos detected Cobl expression specifically in the node and its derivatives until organogenesis stages. Expression was similar in chicken embryos, suggesting COBL has a role in midbrain morphogenesis. invertebrates.