Crooked Neck Pre-mRNA Splicing Factor Like Protein 1 (CRNKL1)

The spliceosome is a ribonucleoprotein complex composed of 5 small nuclear RNAs and more than 50 proteins. CRNKL1, a component of the spliceosome, is a widely expressed pre-mRNA splicing factor.

The deduced 687-amino acid protein contains 16 copies of the conserved tetratricopeptide repeat (TPR) motif, a central homeobox domain, 2 conserved Wxx(F/Y) motifs, and a C-terminal nuclear localization signal. The deduced 687-amino acid protein has a calculated molecular mass of 83 kD. Northern blot analysis detected variable expression of 2.4- and 4.1-kb transcripts in all human tissues and cell lines examined. Western blot analysis revealed bands of 83, 95, and 100 kD, as well as minor bands of 55 to 70 kD.