Cytochrome b5 Reductase 1 (CYB5R1)

The CYB5R1 sequence reported by Zhu et al. (1999), AF169481, maps to 1q32 (build 36.3) (Amberger, 2009).NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction

By searching EST databases for consensus NADPH- and flavin-binding sites, followed by RT-PCR of a hepatoma cell line cDNA library, Zhu et al. (1999) cloned CYB5R1, which they designated B5R.1. The deduced protein contains an N-terminal membrane anchor, followed by 2 FAD-binding motifs and 3 NADPH-binding motifs. Northern blot analysis detected expression of CYB5R1 in virtually all tissues and cell lines examined.