DNA Replication Helicase 2 Like Protein (DNA2L)

DNA2 interacted with mitochondrial DNA polymerase-gamma and significantly stimulated its polymerase activity. DNA2 and flap endonuclease-1 (FEN1) synergistically processed intermediate 5-prime flap structures occurring in DNA replication and long-patch base excision repair (LP-BER) in mitochondria.

The deduced 1,076-amino acid protein has a putative ATP/GTP-binding site motif A and a predicted transmembrane domain. Northern blot analysis detected low expression in KG-1 and HeLa cells and in thymus, but not in any other human tissue examined.Yeast Dna2 helicase is a DNA-dependent ATPase that unwinds duplex DNA to generate single-stranded DNA, which then acts as a template for DNA polymerization during DNA replication.