Dynein, Light Chain, Roadblock Type 2 (DYNLRB2)

Jiang et al. (2001) identified 2 members of the dynein family that they designated DNLC2A and DNLC2B. Both DNLC2A and DNLC2B encode deduced 96-amino acid proteins with molecular masses of 10.9 and 10.8 kD, respectively. The proteins share 77% sequence identity and show high homology to robl/LC7 dynein proteins. Northern blot analysis detected higher expression of DNLC2B than of DNLC2A in all normal tissues tested except liver. Northern blot and RT-PCR analysis in hepatocellular carcinoma (HCC) tissues of 68 Chinese patients showed upregulation of DNLC2A in 45 cases and dowregulation of DNLC2B in 44 cases compared with adjacent tumor-free liver tissues. In 7 additional cancer cell lines studied, DNLC2A was highly expressed in all, whereas DNLC2B was highly expressed in only 1 HCC cell line.