Elongation Factor Tu GTP Binding Domain Containing Protein 2 (EFTUD2)

EFTUD2 is structurally closely related to the eukaryotic translation elongation factor EF2, sharing 27 to 50% sequence identity depending on the region examined. this protein bound GTP specifically. Immunofluorescence microscopy revealed that U5-116kD is located in the nucleus of HeLa cells and colocalizes with snRNP-containing nuclear speckles.

Antibodies against U5-116kD inhibited pre-mRNA splicing in a HeLa nuclear extract. These authors also identified homologs in S. cerevisiae, C. elegans, and mouse, designating them Snu114p, Caeel-116H, and U5-116kD, respectively. Human U5-116kD shares 32%, 74%, and 99% identity with Snu114p, Caeel-116H, and mouse U5-116kD, respectively