Endothelial Overexpressed Lipopolysaccharide Associated Factor 1 (EOLA1)

A novel cDNA, named endothelial-overexpressed lipopolysaccharide-associated factor 1 (EOLA1), EOLA1 is 1404bp long, encoding a 158aa, 17.8kDa protein, mapped to chromosome Xq27.4 with 5 exons, expressed in different human normal tissues and cancer cell lines.

Stable transfection of EOLA1 stimulates ECV304 cell proliferation. The physical interaction of EOLA1 and MT2A may have an important role of cell protection in inflammation reaction.EOLA1 was mainly expressed in E. coli as insoluble inclusion bodies. The protein content in the primary extracted inclusion bodies accounted for over 75%, and it accounted for more than 90% after chromatography and renaturation.