Ets2 Repressor Factor (ERF)

ERF repressed the ETS2 promoter more than 30-fold. ERF also repressed the GATA1 promoter in the presence of its transactivator, GATA1. ERF suppressed the ETS-dependent transforming activity of the gag-myb (18990)-ets fusion oncogene of the avian E26 virus.

The deduced 548-amino acid protein contains an N-terminal ETS domain structurally similar to the ETS domains of ELK1 and SAP1 (ELK4). It also has a central proline-rich region, 2 putative SH3 interaction sites, and several putative phosphorylation sites. Northern blot analysis detected a 2.7-kb transcript in all tissues and cell lines examined. Western blot analysis showed multiple phosphorylated forms of ERF ranging from 75 to 85 kD.