Flavin Adenine Dinucleotide Synthetase 1 (FLAD1)

FAD synthetase (EC 2.7.7.2) catalyzes the adenylation of flavin mononucleotide (FMN) into the redox cofactor FAD.FLAD1 encodes 2 alternatively spliced transcripts, which the authors named FADS1 and FADS2. FADS1 encodes a 587-amino acid protein with a predicted molecular mass of 65.3 kD, and FADS2 encodes a 490-amino acid protein with a predicted molecular mass of 54.2 kD. FADS2 uses a downstream start codon and thus lacks an N-terminal region present in FADS1.

Analysis using protein prediction programs showed that both FLAD1 isoforms have a predicted cytoplasmic location, although FADS1 also contains an N-terminal mitochondrial targeting sequence.both FLAD1 isoforms possess MgCl2-dependent FAD synthetase activity.