G Protein Coupled Receptor 124 (GPR124)

TEM5 was expressed clearly in the endothelial cells of the tumor stroma but not in the endothelial cells of normal colonic tissue. The authors used in situ hybridization to examine Tem5 expression in murine tumors, embryos, and adult tissues.

The N-terminal extracellular region contains 4 simple leucine-rich repeats (LRRs), a C-terminal LRR, an immunoglobulin-type domain, a hormone receptor domain, and a putative G protein-coupled receptor (GPCR) proteolysis site domain typical of class II family members and required for endogenous proteolysis. TEM5 may be a member of the GPCR superfamily involved in transmitting signals across the cell membrane. The mouse ortholog of TEM5 shares 87% amino acid identity with the human protein.