G Protein Regulated Inducer Of Neurite Outgrowth 2 (GPRIN2)

GPRIN2 interacted specifically with G-alpha-o (GNAO1) and G-alpha-z (GNAZ) bound to GTP-gamma-S and GDP-AlF4(-), but did not interact with GDP-bound forms of either G protein subunit. coexpression of GPRIN1 or GPRIN2 with activated G-alpha-o caused formation and extension of neurite-like processes in mouse neuroblastoma cells.The transcript contains repetitive elements in its 3-prime UTR, and the deduced 461-amino acid protein has a calculated molecular mass of 66 kD. RT-PCR of human tissues detected low expression in kidney and lung.The deduced 461-amino acid sequence shares high homology with GPRIN1 and GPRIN3 over the C-terminal region. Northern blot analysis of mouse tissues detected expression in brain only, and Northern blot analysis of human brain regions found expression restricted to the cerebellum.