G Protein Regulated Inducer Of Neurite Outgrowth 3 (GPRIN3)

By database analysis using mouse Gprin1 as probe, Iida and Kozasa (2004) identified human GPRIN3, which they called GRIN3. The deduced 776-amino acid sequence shares high homology with GPRIN1 and GPRIN2 over the C-terminal region. Using purified recombinant GPRIN1 and GPRIN2 expressed in insect cells, as well as immunoprecipitation studies from transfected COS cells, Chen et al. (1999) showed that both GPRIN1 and GPRIN2 interacted specifically with G-alpha-o and G-alpha-z (GNAZ) bound to GTP-gamma-S or GDP-AlF4(-), but did not interact with GDP-bound forms of either G protein subunit. Chen et al. (1999) showed that coexpression of GPRIN1 or GPRIN2 with activated G-alpha-o caused formation and extension of neurite-like processes in mouse neuroblastoma cells.