GINS Complex Subunit 4 (GINS4)

The yeast heterotetrameric GINS complex is made up of Sld5, Psf1 (GINS1), Psf2 (GINS2), and Psf3 (GINS3). The formation of the GINS complex is essential for the initiation of DNA replication in yeast and Xenopus egg extracts.

The deduced 223-amino acid protein contains an N-terminal coiled-coil domain, a central basic region, and a C-terminal domain that is conserved between the mouse and yeast proteins. RT-PCR of mouse tissues detected abundant expression in adult testis, and weak expression in thymus and bone marrow. No Sld5 was detected in brain, lung, liver, spleen, kidney, and heart. Immunohistochemical analysis detected Psf1 and Sld5 in the cytoplasm of transfected NIH3T3 mouse fibroblast.mouse Sld5 and Psf1 interacted in transfected NIH3T3 cells.