GIPC PDZ Domain Containing Family, Member 1 (GIPC1)

GIPC1 protein contains 333 amino acids, including multiple phosphorylation sites and an 80- to 100-amino acid PDZ domain. PDZ domain-containing proteins typically recognize C-terminal amino acids and are involved in protein network signaling. GIPC1 shares 96% amino acid identity with rat Gipc. Northern blot analysis detected a 1.8-kb transcript in all tissues tested, with strongest expression in pancreas, followed by skeletal muscle, brain, kidney, placenta, lung, liver, and lowest expression in heart. Expression levels did not correlate with those of GAIP. Immunoblot analysis demonstrated the presence of GIPC1 primarily in cytosolic fractions but also in membrane fractions. Immunofluorescence analysis showed expression of endogenous GIPC1 in both a diffuse and a punctate staining pattern throughout the cytoplasm.