Glucocorticoid Modulatory Element Binding Protein 1 (GMEB1)

The GMEB1 protein has a KDWK domain, contains sequences that are 95% identical to 3 tryptic peptides of rat GMEB1, and shares 38% identity with rat GMEB2. A rabbit antibody raised against a recombinant GMEB1 fusion protein detected an 85-kD polypeptide in several human and mouse cell lines, in rat liver, and in different mouse tissues. The authors suggested that the difference between the apparent molecular mass of this polypeptide and the calculated molecular mass of 62.5-kD derived from the protein sequence might reflect the high content of acidic residues in GMEB1. By immunofluorescence of HeLa cells expressing recombinant GMEB1, the authors localized the protein to the nucleus. Coimmunoprecipitation experiments confirmed the in vivo interaction of HSP27 with GMEB1.