Golgi Associated PDZ And Coiled Coil Motif Containing Protein (GOPC)

PIST is a PDZ domain-containing Golgi protein. PDZ domains contain approximately 90 amino acids and bind the extreme C terminus of proteins in a sequence-specific manner.

The deduced 454-amino acid human protein, which is approximately 92% identical to the mouse protein, contains 2 putative coiled-coil domains, with a potential leucine zipper in the second domain, followed by a novel region conserved in mouse and worm, and a PDZ domain. Northern blot analysis revealed ubiquitous expression of a 4.4-kb transcript, with highest expression in brain and heart.Coimmunoprecipitation and colocalization experiments demonstrated that the specific interaction of Grid2 and nPist requires the C terminus of Grid2 and the nPist PDZ domain.