Golgin A7 (GOLGA7)

GCP16 contains a short coiled-coil domain. Northern blot analysis detected a 2.0-kb transcript in all tissues examined, with abundant expression in testis, ovary, and spleen. A similarity search revealed GCP16 homologs in mouse, nematode, and fly, but not in yeast and plants. Immunoelectron microscopy detected GCP16 associated with the Golgi stack and related structures. SDS-PAGE detected endogenous GCP16 at an apparent molecular mass of 16 kD.

DHHC9 and GCP16 coimmunoprecipitated in transfected human embryonic kidney cells. Following expression in insect cells, the DHHC9/GCP16 complex showed protein acyltransferase activity, leading to incorporation of radioactive palmitate into both DHHC9 and an HRAS substrate.