Guanine Nucleotide Binding Protein Like Protein 3 (GNL3)

GNL3 was not found in the differentiated cells of adult tissue, suggesting a role in maintaining stem cell self-renewal. Depletion or overexpression of nucleostemin reduces cell proliferation in CNS stem cells and transformed cells. Mutation analysis indicated that excessive nucleostemin, particularly mutants lacking the GTP-regulatory domain, prevents cells from entering mitosis and causes apoptosis in a p53-dependent manner. The N-terminal basic domain mediates the interaction with p53 and is required for the cell death caused by overexpression.The mouse and rat proteins share 90% sequence identity, and the 549-amino acid human protein shares 81% sequence identity with the rat protein. The NS protein contains 2 GTP-binding motifs, an N-terminal basic domain, and a C-terminal acidic domain.