Guanine Nucleotide Binding Protein Subunit Beta 2 Like Protein 1 (GNb2L1)

RACK1 is a major component of translating ribosomes, which harbor significant amounts of PKC. Loading 60S subunits with EIF6 caused a dose-dependent translational block and impairment of 80S formation, which were reversed by expression of RACK1 and stimulation of PKC in vivo and in vitro. PKC stimulation led to EIF6 phosphorylation, and mutation of a serine residue in the carboxy terminus of EIF6 impaired RACK1/PKC-mediated translational rescue.

The deduced 317-amino acid protein shares significant similarity with GNB2, including an identical structure of 7 homologous segments and a number of identical or isofunctional consensus residues. Northern blot analysis detected a 1.3-kb transcript abundantly expressed in B-lymphoblastoid cells.