Guanosine Monophosphate Reductase 2 (GMPR2)

GMPR2 protein was able to reduce GMP. Transfection experiments demonstrated that overexpression of GMPR2 promoted the monocytic differentiation of HL-60 leukemia cells.The deduced 348-amino acid protein has a calculated molecular mass of 37.9 kD and shares 90% sequence identity with GMPR1 and 69% identity with E. coli GMPR. Northern blot analysis detected relatively high levels of both GMPR1 and GMPR2 in heart, skeletal muscle, and kidney, and relatively low levels of both in colon, thymus, and peripheral blood leukocyte. Strong signals of GMPR2 were detected in brain, liver, and placenta, whereas weak signals of GMPR1 were observed in these tissues. The apparent Km of GMPR2 for NADPH and GMP are 26.6 and 17.4 microM, respectively.