HBV XAg Transactivated Protein 12 (XTP12)

C6orf62, a gene whose protein expression and function has not been described to date. The gene product of C6orf62 was designated pituitary associated regulator of hormone secretion (PARS).

Initial gene expression screens showed that PARS transcription is differentially regulated in secreting and non-secreting pituitary adenomas, as well as in normal pituitary tissue. Inspired by these findings, C6orf62 was amplified by PCR from HEK293 cDNA using specific primers. To investigate the gene product of C6orf62, it was ligated into an expression vector with an N-terminal His-tag and expressed in E. coli. Western blotting using an anti-His antibody confirmed the expression of a 30 kDa recombinant His-tagged protein from C6orf62.