HIV1 Rev Binding Protein (HRB)

HRB is related to nucleoporins, a class of proteins that mediate nucleocytoplasmic transport. This encoded protein binds the Rev activation domain when Rev is assembled onto its RNA target and can significantly enhance Rev activity when overexpressed. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.A partial clone from the initial screen was used to obtain a full-length 2.4-kb RIP cDNA. The authors found that the predicted 562-amino acid protein is related to the nucleoporins, a class of proteins that mediate nucleocytoplasmic transport. The protein was detected in the nuclear extract of HeLa cells and by immunofluorescence on the nuclear surface.