HECT Domain And RCC1 Like Domain Protein 1 (HERC1)

The HERC1 protein contains both an N-terminal and a C-terminal RCC1-like domain (RLD), 7 WD domains characteristic of the beta subunit of heterotrimeric G proteins thought to be involved in protein-protein interactions, 3 putative SH3 binding sites, 7 polar amino acid regions, a putative leucine zipper, and a C-terminal HECT domain. Northern blot analysis detected a 15-kb HERC1 transcript expressed ubiquitously in all human and mouse tissues examined, with the highest levels in testis and brain and the lowest in liver.

HERC1 was overexpressed in all human tumor cell lines tested, independent of their developmental origin. Subcellular localization studies indicated that HERC1 is located in the cytosol and the Golgi apparatus.