HECT Domain And RCC1 Like Domain Protein 5 (HERC5)

CEB1; CEBP1; E3 ISG15 protein ligase HERC5; Cyclin-E-binding protein 1

HECT Domain And RCC1 Like Domain Protein 5 (HERC5)
HERC5 is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets. The gene lies in a cluster of HERC family genes on chromosome 4.
Immunoprecipitation and immunoblot analysis confirmed that HERC5 specifically copurified with ISG15. RT-PCR analysis detected upregulated HERC5 expression after IFNB treatment. Suppression of HERC5 using small interfering RNA showed that HERC5 was required for the ISG15 conjugation system (ISGylation) following IFNB treatment.

Organism species: Homo sapiens (Human)