Hypermethylated In Cancer 2 (HIC2)

The deduced full-length HIC2 protein contains 615 amino acids. It has an N-terminal BTB/POZ domain, a conserved GLDLSKK/R polypeptide, and 5 C-terminal Kruppel-type C2H2 fingers, 4 of which are grouped and are separated by a typical 7- to 8-amino acid link. HIC2 shares more than 80% homology with HIC1 through the BTB/POZ and zinc finger domains, and both proteins have identical GLDLSKK/R motifs.

HIC2 colocalized with HIC1 onto nuclear dots. Their BTB/POZ domains heterodimerized and repressed transcription from a reporter plasmid. Inhibitor studies indicated that HIC1 and HIC2 do not recruit HDAC-containing complexes. The central GLDLSKK/R motif was found to interact with C-terminal-binding protein .