Interferon Related Developmental Regulator 1 (IFRD1)

The deduced 453-amino acid human IFRD1 protein is 90% identical to both rat and mouse Ifrd1 (Pc4), and 47.6% identical to human SKMc15 (IFRD2). The C terminus of IFRD1 shows 47.5% similarity to the entire human interferon-gamma (IFNG) protein. IFRD1 has a calculated molecular mass of 50,685 Da. Northern blot analysis detected a 2.2-kb IFRD1 transcript in several human tissues, although at different levels of expression. At midgestation during mouse development, Ifrd1 was expressed primarily in specific differentiating structures and body organs.

In unpolarized cells, the level of Tis7 protein was elevated and Tis7 translocated into the nucleus. cDNA microarray analysis revealed that overexpression of Tis7 repressed several genes.