Intraflagellar Transport Protein 122 (IFT122)

WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. The full-length WDR10 cDNA encodes a deduced 1,242-amino acid protein with an AF2-like domain, 7 N-terminal WD repeat units, and 2 potential nuclear localization sequences. Northern blot analysis detected a single 4-kb WDR10 transcript in most tissues tested, with predominant expression in testis and pituitary; a 3.5-kb transcript was detected in HL60 leukemia cells. Study of a WDR10-GFP fusion protein demonstrated that WDR10 is localized in the cytoplasm. In situ hybridization on reproductive tissues in the rat showed stage-specific expression of WDR10 within developing sperm and ovarian follicles.