Intraflagellar Transport Protein 74 (IFT74)

CMG1 was 1 of several transcripts upregulated by umbilical vein endothelial cells during capillary morphogenesis in 3-dimensional collagen matrices.deduced 600-amino acid protein has a calculated molecular mass of 65 kD. CMG1 contains a coiled-coil domain and several putative phosphorylation sites. The coiled-coil domain shares 24% identity with myosin heavy chain sequences from various species. RT-PCR detected highest expression in adult and fetal kidney and lower expression in adult heart, placenta, lung, liver, and pancreas, and in fetal heart, lung, and liver. Little to no expression was detected in adult brain and skeletal muscle or in fetal brain, thymus, and spleen. Transfection into epithelial tumor cells revealed targeting of fluorescent-tagged CMG1 to an intracellular vesicular compartment.