Ischemia/Reperfusion Inducible Protein (IRIP)

YRDC shares 90% amino acid identity with its mouse ortholog and is highly conserved over its SUA5-YCIO-YRDC domain from E. coli to human. Northern blot analysis detected a 2.5-kb transcript in all human tissues examined with highest expression in liver, brain, and pancreas.YRDC transfection and overexpression resulted in decreased 1-methyl-4-phenylpyridinium (MPP+) transport, and a YRDC-mutant protein resulted in increased MPP+ transport. Inhibition of YRDC by short interfering RNA in HeLa cells resulted in increased MPP+ transport, and YRDC overexpression decreased MPP+ transport mediated by organic cation transporters OCT2 (SLC22A2) and OCT3 (SLC22A3), as well as by organic anion transporter OAT1 (SLC22A6) and monoamine transporters DAT (SLC6A3), NET (SLC6A2), and SERT (SLC6A4).