Kinesin Light Chain 2 (KLC2)

KLC2has an N-terminal coiled-coil region of about 100 amino acids and 6 imperfect tetratricopeptide repeats of about 34 amino acids each. Northern and Western blot analyses detected Klc2 in all mouse tissues examined, with enrichment in central and peripheral neuronal tissues. Immunofluorescence analysis of cultured rat hippocampal precursor cells showed that Klc2 levels were not affected by differentiation. In situ hybridization of mouse brain showed that both Klc1 and Klc2 were enriched in olfactory bulb, hippocampus, dentate gyrus, and the granular layer of cerebellum. Within sciatic nerve, Klc2 was expressed in Schwann cells and localized within axons in a punctate pattern. Fractionation of whole mouse brain extracts revealed Klc1 and Klc2 in the cytosolic fraction and Klc2 in the microsomal fraction.