Kinetochore Associated Protein 1 (KNTC1)

KNTC1 is a homolog of the Drosophila 'rough deal' (Rod) protein, a component of the mitotic checkpoint surveillance mechanism. This complex prevents the onset of anaphase by elaborating an inhibitory signal until all chromosomes are properly attached and aligned on the spindle.

KNTC1 throughout the cytoplasm of cells at all mitotic stages. A fraction of the total pool was associated with kinetochores and the spindle during mitosis. KNTC1 initially concentrated at kinetochores during prometaphase. During metaphase, staining moved to the spindle fibers and prominently to the spindle poles. By late anaphase, KNTC1 was detected only at the spindle poles. KNTC1 colocalized with ZW10 at kinetochores at prometaphase.