LMBR1 Domain Containing Protein 1 (LMBRD1)

The protein encoded by LMBRD1, which the authors called LMBD1, contains 540 amino acids and has a calculated molecular mass of 61.4 kD. It contains 9 predicted transmembrane domains and 6 putative N-glycosylation sites. In transfected HeLa cells and primary human fibroblasts, fluorescence-tagged LMBD1 exhibited a punctate distribution throughout the cytoplasm and perinuclear region that colocalized with a lysosomal marker.

Electron microscopy and immunogold labeling showed that LMBD1 localized to the lysosomal membrane. Analysis of LMBD1 glycosylation status confirmed that LMBD1 has 9 transmembrane helices, with an N terminus in the lysosomal interior and a cytoplasmic C terminus.