Lantibiotic Synthetase Component C Like Protein 2 (LANCL2)

The deduced 450-amino acid protein contains a Walker A box and 7 repetitive hydrophobic regions that contain GxxG motifs. It shares 57.9% amino acid identity with LANCL1. The 1.8-kb transcript was highly expressed in testis. All other tissues examined showed weak expression of the 4.1- and 1.8-kb transcripts. RNA dot blot analysis confirmed ubiquitous LANCL2 expression, with highest levels in amygdala, followed by temporal lobe and testis. Database analysis revealed conservation of LANCL proteins in bacteria, plant, and animal genomes.LANCL2 exhibited Zn(2+)-dependent glutathione binding. LANCL2 bound the SH3 domain of mouse Eps8, but not other SH3-containing proteins. The interaction between LANCL2 and Eps8 did not require either of the PxxP motifs of LANCL2, but it was inhibited by glutathione.