Leucine Carboxyl Methyltransferase 1 (LCMT1)

LCMT1 catalyzes the methylation of the carboxyl group of the C-terminal leucine residue (leu309) of the catalytic subunit of protein phosphatase-2A (PPP2CA). The deduced 334-amino acid LCMT1 protein has a predicted molecular mass of 38.305 kD and shares high sequence similarity in the methyltransferase domain with other leucine carboxyl methyltransferases across diverse species.LCMT1 is the major PP2A-methylating enzyme. LCMT1 knockdown also decreased association of the PP2A regulatory subunit B-alpha with the PP2A catalytic subunit, induced apoptosis, and sensitized HeLa and colon carcinoma cells to the microtubule spindle-targeting drug nocodazole.LCMT1 is important for normal progression through mitosis and for cell survival.