Leucine Rich Repeat And Fibronectin Type III Domain Containing Protein 1 (LRFN1)

C-terminal Lrfn1 bound PSD95 (DLG4) by immunoprecipitation and GST pull-down assays. Cotransfection of PSD95 and Lrfn1 in HEK293 cells showed localization of PSD95 to the cell periphery overlapping with Lrfn1 distribution.The deduced LRFN1 protein contains a predicted signal peptide, a leucine-rich N-terminal domain, 6 leucine-rich repeat domains, a leucine-rich C-terminal domain, Ig C2-like domain, a fibronectin type III domain, transmembrane region, and an ESTV PDZ-binding motif. The LRFN family members share similarity with proteins of the Slit family, the Amigo family (AMIGO1), and the Nogo66 receptor (RTN4R).LRFN1 showed strong expression in specific brain regions tested, including amygdala, cerebellum, hippocampus, substantia nigra, subthalamic nucleus, thalamus, and caudate nucleus.