Leucine Rich Repeat And Sterile Alpha Motif Containing Protein 1 (LRSaM1)

LRSAM1 is a multifunctional RING finger protein that selectively regulates cell adhesion molecules, has ubiquitin ligase activity, and plays a role in receptor endocytosis and viral budding.The deduced 723-amino acid protein contains an LRR, ezrin-radixin-moesin (ERM) domain, a coiled-coil region, a SAM domain, 2 PTAP motifs, and a C-terminal RING finger domain. The deduced 702-amino acid protein has a predicted molecular mass of 80 kD and contains a leucine-rich repeat (LRR) motif, nuclear localization signal, leucine zipper domain, and a ring zinc finger domain. Northern blot analysis detected a 3.14-kb transcript in human heart, skeletal muscle, kidney, liver, and in all brain regions examined. Western analysis localized RIFLE to the cytosol and nuclear fractions when expressed in PC12 cells.