Dynein Assembly Factor 1, Axonemal (DNAAF1)

LRRC50 is a component of the microtubule outer dynein arm and appears to stabilize microtubule-based cilia and actin-based brush border microvilli . The deduced proteins contain 637 and 725 amino acids, and both have 6 N-terminal LRR motifs, an LRR cap, a coiled-coil, and a nonconserved proline-rich domain. The sixth LRR partially overlaps the putative LRR cap. In situ hybridization of zebrafish showed that Lrcc50 was expressed in ciliated tissues throughout development, including dorsal forerunner cells during gastrulation and otic placode, pronephric duct, floorplate, neural tube, nose, and brain (diffusely) during later stages. Fluorescence-tagged LRRC50 localized to spindle poles of mitotic human embryonic kidney cells and MDCK canine kidney cells and to ciliary structures extending from the basal body of ciliated MDCK cells.