Leucine Zipper, Putative Tumor Suppressor 2 (LZTS2)

The 2.0-kb transcript contained the expected sequence from all coding exons; transcripts of 1.7 kb and 1.35 kb lacked exon 4 and exon 3, respectively. Northern blot analysis using an LZTS2 exon 3 probe detected a 2.8-kb transcript in all tissues examined, with highest expression in prostate and testis, followed by spleen, small intestine, uterus, and thymus, and lowest expression in peripheral blood lymphocytes. A minor 3.8-kb transcript detected may contain additional 5-prime noncoding sequence. Northern blot analysis using the complete cDNA detected multiple LZTS2 isoforms in normal tissues, consistent with alternative splicing, and LZTS2 expression was absent in some prostate cancer cell lines, while in other prostate cancer cells lines the ratio of major to minor transcripts was significantly altered