Lin-54 Homolog (LIN54)

LIN54 is a component of the LIN, or DREAM, complex, an essential regulator of cell cycle genes . The deduced 749-amino acid protein contains 2 cysteine-rich C-terminal CxC domains separated by a short spacer. SDS-PAGE of MOLT-4 cells and Western blot analysis of HeLa cells detected LIN54 at an apparent molecular mass of 110 kD.Domain analysis revealed that the C-terminal helix-coil-helix region of LIN54 was essential for its binding to p130 (RBL2) and BMYB.

The CxC domain of LIN54 was identified as a DNA-binding domain that could bind the promoter region of the CDC2 gene in a sequence-specific manner. LIN54 bound 2 regions in the CDC2 promoter, 1 of which overlaps with the cell cycle homology region at the transcription start site.