Lysine Specific Demethylase 5A (KDM5A)

KDM5A is a ubiquitously expressed nuclear protein. It binds directly, with several other proteins, to retinoblastoma protein which regulates cell proliferation. This protein also interacts with rhombotin-2 which functions distinctly in erythropoiesis and in T-cell leukemogenesis. Rhombotin-2 is thought to either directly affect the activity of the encoded protein or may indirectly modulate the functions of the retinoblastoma protein by binding to this protein. The deduced 1,722-amino acid protein has a calculated molecular mass of 196 kD. RBP2 contains a potential N-terminal zinc finger motif, a central region with similarity to the engrailed family of homeotic genes, and a C-terminal RB-binding motif. Endogenous RBP2 migrated at an apparent molecular mass of 195 kD. Immunoprecipitation of fractionated glioblastoma cells indicated that RBP2 is a nuclear protein.