MICAL C-Terminal Like Protein (MICALCL)

The deduced 680-amino acid protein has a calculated molecular mass of 75 kD. Northern blot analysis detected Ebitein1 expression in mouse testis, but not in 12 other mouse tissues examined, including brain. Western blot analysis detected Ebitein1 at an apparent molecular mass of 75 kD in testis only. Immunohistochemical analysis showed high Ebitein1 expression in round spermatids, but not in other testicular cells. During spermatogenesis, Ebitein1 first appeared after meiosis when cells became haploid, then the amount of Ebitein1 gradually increased, reaching a maximum at Oakberg stage 9. Subsequently, the level of Ebitein1 decreased such that it was undetectable when the flagellum of the spermatozoon was generated. On a subcellular level, Ebitein1 localized in the cytoplasm.