Mannosidase Alpha Class 2A Member 2 (MAN2A2)

A 5-kb cDNA clone of the MANA2X gene was then isolated from a human melanoma cDNA library. Comparison between the MANA2X and MANA2 cDNAs suggested that the cloned cDNA encodes a truncated polypeptide with 796 amino acid residues, while the product of the MANA2 gene consists of 1144 amino acid residues.

However, further studies indicated that alternative splicing of the MANA2X transcript can result in an additional transcript encoding an 1139-amino acid polypeptide. Northern analysis showed transcription of MANA2X in various tissues, suggesting that it is a housekeeping gene. COS cells transfected with MANA2X cDNA containing the full-length open reading frame showed an increase in alpha-mannosidase activity.